Telefon ve e-posta Desteği
Sıkça Sorulan Sorular
Yazarlar İçin Teknik Bilgiler
İletişim Bilgileri
Ana Sayfaya Dönüş
 

Kongre Takvimi
Buradaki bilgiler JournalAgent™ online dergicilik sisteminden alınmaktadır.

The Anatolian Journal of Cardiology

[Anatol J Cardiol]
Anatol J Cardiol. 2016; 16(2): 76-83 | DOI: 10.5152/akd.2015.5925  

Effect of intermittent hypoxia on the cardiac HIF-1/VEGF pathway in experimental type 1 diabetes mellitus

Derya Güzel1, Ali Doğan Dursun2, Hakan Fıçıcılar2, Demet Tekin2, Ayhan Tanyeli4, Fırat Akat2, Ferda Topal Çelikkan3, Bizden Sabuncuoğlu3, Metin Baştuğ2
1Department Of Physiology, Faculty Of Medicine, Sakarya University; Sakarya- Turkey
2Department Of Physiology, Faculty Of Medicine, Ankara University; Ankara- Turkey
3Histology And Embryology, Faculty Of Medicine, Ankara University; Ankara- Turkey
4Department Of Physiology, Faculty Of Medicine, Atatürk University; Erzurum- Turkey

Objective: High altitude and hypoxic preconditioning have cardioprotective effects by increasing coronary vascularity, reducing post-ischemic injury, and improving cardiac function. Our purpose was to examine if intermittent hypoxia treatment has any restoring effects related to the possible role of the HIF-1/VEGF pathway on diabetic cardiomyopathy.
Methods: Wistar Albino male rats (n=34) were divided into four groups: control (C), intermittent hypoxia (IH), diabetes mellitus (DM), and diabetes mellitus plus intermittent hypoxia (DM+IH). Following a streptozotocin (STZ) injection (50 mg/kg, i.p.), blood glucose levels of 250 mg/dL and above were considered as DM. IH and DM+IH groups were exposed to hypoxia 6 h/day for 42 days at a pressure corresponding to 3000 m altitude. Twenty-four hours after the IH protocol, hearts were excised. Hematoxylin and eosin-stained apical parts of the left ventricles were evaluated. Hypoxia inducible factor-1 (HIF-1), vascular endothelial growth factor 164 (VEGF164), and VEGF188 polymerase chain reaction products were run in agarose gel electrophoresis. Band density analysis of UV camera images was performed using Image J. The data were compared by one-way ANOVA, repeated measures two-way ANOVA, and the Kruskal-Wallis test.
Results: The percent weight change was lower in the DM group than in the controls (p=0.004). The tissue injury was the highest in the DM group and the least in the IH group. Diabetes decreased, whereas the IH treatment increased the vascularity. A decrease was observed in the VEGF188 mRNA levels in the DM+IH group compared with the C group, but there were no difference in HIF-1α and VEGF164 mRNA levels between the groups.
Conclusion: The IH treatment restored the diabetic effects on the heart by reducing tissue injury and increasing the capillarity without transcriptional changes in HIF-1/VEGF correspondingly.

Keywords: angiogenesis, diabetic cardiomyopathy, intermittent hypoxia, HIF-1, VEGF


Derya Güzel, Ali Doğan Dursun, Hakan Fıçıcılar, Demet Tekin, Ayhan Tanyeli, Fırat Akat, Ferda Topal Çelikkan, Bizden Sabuncuoğlu, Metin Baştuğ. Effect of intermittent hypoxia on the cardiac HIF-1/VEGF pathway in experimental type 1 diabetes mellitus. Anatol J Cardiol. 2016; 16(2): 76-83

Sorumlu Yazar: Ali Doğan Dursun, Türkiye


ARAÇLAR
Tam Metin PDF
Yazdır
Alıntıyı İndir
RIS
EndNote
BibTex
Medlars
Procite
Reference Manager
E-Postala
Paylaş
Yazara e-posta gönder

Benzer makaleler
PubMed
Google Scholar




 

 
 

Online Kongre Hizmetleri
Kongre Web Sitesi
Bildiri Özeti Toplama ve Değerlendirme
Online Kayıt ve Konaklama
Elektronik Poster (e-poster)
Video Podcast, WebCast
Bilimsel Program Düzenleyici
Bildiri Özeti ve Tam Metin CD'leri
 

Online Kurumsal Hizmetler
Dernekler İçin Web Siteleri
Hakemli Dergiler İçin Online Makale Toplama
   ve Değerlendirme

Uluslararası Dizin Danışmanlığı
PubMed®, LinkOut® Hizmetleri
Online Çalışma Grupları
 

Referans
Çalıştığımız Online Hakemli Dergiler
Destek Verdiğimiz Kongreler
Yazarlar İçin Teknik Bilgiler

İletişim
Bize Ulaşın
LookUs

 
 
1999 - 2019 © LookUs Scientific

Bu sitede sözü edilen uygulamalara ait teknoloji, uygulama metodu, ekran görünüşleri; ticari marka, ticari görünüm, kanun dışı rekabet ve başka kanunlarca; yerel ve uluslararası copyright düzenlemeleri ile korunmuştur. Site üzerindeki herhangi bir metin ve imaj LookUs Bilişim Hizmetleri Ltd.'nin açık ve net izni olmadan kopyalanamaz veya dağıtılamaz.
Metinler ve şekiller içinde geçen gerçek şirket ve ürün adları, ilgili firmaların ticari markaları olabilir.